黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征

doi:10.7522/j.issn.1000-0240.2013.0053

冰川冻土 ›› 2013, Vol. 35 ›› Issue (2): 447-456.doi: 10.7522/j.issn.1000-0240.2013.0053

黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征

毛文梁¹, 台喜生², 伍修锟², 刘光琇², 陈拓², 陈年来¹, 张威², 王筠², 龙昊知², 张宝贵², 张勇³, 高海宁³

1. 甘肃农业大学资源与环境学院, 甘肃兰州 730070;
2. 中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃兰州 730000;
3. 河西学院, 甘肃张掖 734000

收稿日期:2012-10-06 修回日期:2012-12-28 出版日期:2013-04-25 发布日期:2013-05-14
通讯作者: 陈年来,E-mail:chennl@gsau.edu.cn E-mail:chennl@gsau.edu.cn
作者简介:毛文梁(1987-), 女, 山西朔州人, 2009年毕业于甘肃农业大学, 现为甘肃农业大学在读硕士研究生, 主要从事植物生态学及环境微生物研究.
基金资助:
国家自然科学基金项目(91025002; 31070357; 31100365); 中国科学院重点部署项目(KZZD-EW-04-05)资助

Altitudinal Variation Characteristics of the Cultivable Soil Bacterial Communityon the Upper Reaches of the Heihe River, Qilian Mountains

MAO Wen-liang¹, TAI Xi-sheng², WU Xiu-kun², LIU Guang-xiu², CHEN Tuo², CHEN Nian-lai¹, ZHANG Wei², WANG Yun², LONG Hao-zhi², ZHANG Bao-gui², ZHANG Yong³, GAO Hai-ning³

1. College of Resource and Environmental Sciences, Gansu Agricultural University, Lanzhou Gansu 730070, China;
2. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering ResearchInstitute, Chinese Academy of Sciences, Lanzhou Gansu 730000, China;
3. Hexi University, Zhangye Gansu 734000, China

Received:2012-10-06 Revised:2012-12-28 Online:2013-04-25 Published:2013-05-14

摘要/Abstract

摘要：

利用PYGV、 R2A、 NB和Czapek 4种培养基, 研究了不同海拔下黑河上游祁连山区土壤细菌群落结构的变化规律.结果表明: 可培养细菌数量为4.6×10⁶~37.0×10⁶CFU·g^-1, 随海拔升高明显减少; 基于16S rRNA基因序列分析共发现了7个门、 19个属、 26种细菌, 其中Agreia pratensis, Mucilaginibacter ximonensis, 嗜冷冷杆菌(Cryobacterium psychrophilum)和氧化节杆菌(Arthrobacter oxydans)四种细菌是优势种; 嗜冷冷杆菌的相对丰度在高海拔地区明显增加, Agreia pratensis的相对丰度随海拔升高而降低; 细菌的多样性随海拔升高呈现出先升高后降低的趋势. 冗余分析(RDA)显示, 可培养细菌数量与海拔呈显著负相关, 细菌的多样性与植被指数和土壤理化性质均存在明显的相关关系, 说明可培养细菌数量主要受海拔的影响, 而植被和土壤理化性质是影响细菌群落多样性的主要因素.

关键词: 黑河上游, 祁连山, 海拔, 可培养细菌, 植被

Abstract:

The upper reaches of the Heihe River are located in the Qilian Mountains on the eastern edge of the Tibetan Plateau. There are many reports on the hydrology and vegetation of the reaches, but less of them involve the distribution of soil bacterial communities. In this paper, soil samples were collected at different altitudes (2 814~4 130 m above sea level) from the upper reaches of the Heihe River. Cultivable soil bacteria were investigated with PYGV, R2A, NB and Czapek agar mediums. It is found that the number of cultivable bacteria ranges from 4.6 to 37.0 (10⁶ CFU·g^-1). Population fluctuation of bacteria is significant along with altitudes(P<0.05). From the soil 26 species were isolated by sequencing PCR-amplified 16S rDNA. These species are affiliated with 19 genera belonging to α-Proteobacteria,β-Proteobacteria,γ-Proteobacteria,Actinobacteria,Bacteroides,Firmicutes and Verrucomicrobia. Among them, Agreia pratensis, Mucilaginibacter ximonensis, Cryobacterium psychrophilum and Arthrobacter oxydans are dominant species. The relative abundance of Cryobacterium psychrophilum obviously concentrates at high altitude, but that of Agreia pratensis decreases with altitude. Statistical analysis shows that altitude and vegetation type are the principal environmental factors in the upper reaches of the Heihe River, which affect the distribution of bacterial community. Cultivable number of bacteria has negative correlation with altitude, and the diversity of bacteria increases firstly and then decreases with altitude, which is primarily controlled by plant biomass and cover degree. Besides, the diversity of cultivable bacteria is also correlated with some soil physicochemical properties (e.g., organic material, total organic carbon, salt and available K).

Key words: upper reaches of the Heihe River, Qilian Mountains, altitude, cultivable bacteria, vegetation

中图分类号:

Q938.1

毛文梁, 台喜生, 伍修锟, 刘光琇, 陈拓, 陈年来, 张威, 王筠, 龙昊知, 张宝贵, 张勇, 高海宁. 黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征[J]. 冰川冻土, 2013, 35(2): 447-456.

MAO Wen-liang, TAI Xi-sheng, WU Xiu-kun, LIU Guang-xiu, CHEN Tuo, CHEN Nian-lai, ZHANG Wei, WANG Yun, LONG Hao-zhi, ZHANG Bao-gui, ZHANG Yong, GAO Hai-ning. Altitudinal Variation Characteristics of the Cultivable Soil Bacterial Communityon the Upper Reaches of the Heihe River, Qilian Mountains[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2013, 35(2): 447-456.

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黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征

Altitudinal Variation Characteristics of the Cultivable Soil Bacterial Communityon the Upper Reaches of the Heihe River, Qilian Mountains

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