祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究

doi:10.7522/j.issn.1000-0240.2013.0085

冰川冻土 ›› 2013, Vol. 35 ›› Issue (3): 751-760.doi: 10.7522/j.issn.1000-0240.2013.0085

祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究

张淑红¹, 侯书贵^2,3, 秦翔³, 杜文涛³, 孙维君⁴, 闫沛迎³

1. 商丘师范学院生命科学学院, 河南商丘476000;
2. 南京大学地理与海洋学院, 江苏南京210093;
3. 中国科学院寒区旱区环境与工程研究所冰冻圈科学国家重点实验室, 甘肃兰州730000;
4. 山东师范大学人口·资源与环境学院, 山东济南250358

收稿日期:2012-12-20 修回日期:2013-03-11 出版日期:2013-07-25 发布日期:2013-07-25
通讯作者: 侯书贵,E-mail:shugui@nju.edu.cn E-mail:shugui@nju.edu.cn
作者简介:张淑红(1977- ), 女, 甘肃庆城人, 副教授, 2007年在中国科学院寒区旱区环境与工程研究所获博士学位, 现从事环境微生物及其应用研究.E-mail: mariazhangsh@yahoo.com.cn
基金资助:
国家自然科学基金项目(31100369; 41201067; 41071046; 41171052); 冰冻圈科学国家重点实验室自主项目(SKLCS-ZZ-2012-01-05)资助

Preliminary Research on the Dominant Bacterial Population Affected by Retreat of the Laohugou Glacier No. 12 in the Qilian Mountain

ZHANG Shu-hong¹, HOU Shu-gui^2,3, QIN Xiang³, DU Wen-tao³, SUN Wei-jun⁴, YAN Pei-ying³

1. College of Life Science, Shangqiu Normal University, Shangqiu Henan 476000, China;
2. School of Geographic and Oceanographic Sciences, Nanjing University, Nanjing Jiangsu 210093, China;
3. State Key Laboratory of Cryospheric Sciences, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou Gansu 730000, China;
4. College of Population, Resources and Environment, Shandong Normal University, Ji'nan Shandong 250014, China

Received:2012-12-20 Revised:2013-03-11 Online:2013-07-25 Published:2013-07-25

摘要/Abstract

摘要：

随着全球冰川正在越来越多的地区融化, 冰川微生物资源很可能会由于冰川退缩而未被人类所发现就已受到生存的威胁而濒危.以祁连山老虎沟12号冰川消融区和末端雪样及末端土样为研究对象, 采用培养方法、分子鉴定, 研究冰川雪样优势菌群在冰川末端土样的分布状况及生理生化指标变化情况, 分析冰川细菌优势菌群在冰川退缩后适应非雪环境的能力.结果表明: 冰川末端雪样优势菌为1BW1和1BW2所代表的Pedobacter, 该属在冰川消融区雪样和冰川末端土样中未分离到; 冰川消融区雪样优势菌为2BW所代表的Acinetobacter, 该属在冰川末端雪样中的数量较少, 在冰川末端土样中的数量更少.不同采样位点16S rRNA序列相似性高的菌株其生理生化特征比相同采样位点的大.因此, 冰川冰退缩可能会引发冰川雪样中的优势种群不能适应新环境而灭绝. 应加强冰川细菌资源利用和保护的研究基础.

关键词: 老虎沟12号冰川, 退缩, 细菌优势种群

Abstract:

Global glaciers are ablating with the maximal rate on record in more and more areas all over the world. This phenomenon determines the potential loss of glacial microbial resources without being discovered. Taken snow samples from the glacial ablation zone and terminus, soil samples from glacial terminus as research objects, the variation of dominant bacterial community in glacial snow with adaptation to non-snow environment in response to the retreat of Laohugou Glacier No. 12 in the Qilian Mountains was investigated, using culture and molecular methods, as well as physiological and biochemical indexes. The result demonstrates that the dominant population of Pedobacter, represented by 1BW1 and 1BW2, in snow sample from the glacial terminus is unable to isolate from snow sample from the glacial ablation zone and soil sample from the glacial terminus. Moreover, dominant population of Acinetobacter, represented by 2BW, in the snow sample from the glacial ablation zone is more than that in the snow sample from glacial terminus and is less in the soil sample from the glacial terminus. Strains with high 16S rRNA sequence similarity in different sampling sites are more different in physiological and biochemical characteristics. Therefore, the dominant populations in glacial snow may probably become extinction owing to unable to adapt the new environment after glacial retreat. The research is a basis for the utilization and protection of bacterial resources in glaciers.

Key words: Laohugou Glacier No. 12, retreat, dominant bacterial population

中图分类号:

Q938.1+5

张淑红, 侯书贵, 秦翔, 杜文涛, 孙维君, 闫沛迎. 祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究[J]. 冰川冻土, 2013, 35(3): 751-760.

ZHANG Shu-hong, HOU Shu-gui, QIN Xiang, DU Wen-tao, SUN Wei-jun, YAN Pei-ying. Preliminary Research on the Dominant Bacterial Population Affected by Retreat of the Laohugou Glacier No. 12 in the Qilian Mountain[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2013, 35(3): 751-760.

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祁连山老虎沟12号冰川退缩对细菌优势种群影响的初步研究

Preliminary Research on the Dominant Bacterial Population Affected by Retreat of the Laohugou Glacier No. 12 in the Qilian Mountain

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