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冰川冻土 ›› 2013, Vol. 35 ›› Issue (3): 761-769.doi: 10.7522/j.issn.1000-0240.2013.0086

• 寒旱区生物学 • 上一篇    下一篇

天山乌鲁木齐河源1号冰川前沿冻土活动层古菌群落的垂直分布格局

顾燕玲1, 史学伟2, 祝建波1, 倪永清2,3, 程国栋3   

  1. 1. 石河子大学生命科学学院, 新疆 石河子 832000;
    2. 石河子大学食品学院, 新疆 石河子 832000;
    3. 中国科学院寒区旱区环境与工程研究所冻土工程国家重点实验室, 甘肃 兰州 730000
  • 收稿日期:2013-01-13 修回日期:2013-05-05 出版日期:2013-07-25 发布日期:2013-07-25
  • 通讯作者: 倪永清,E-mail:niyqlzu@sina.com;程国栋,E-mail:gdcheng@lzb.ac.cn E-mail:niyqlzu@sina.com;gdcheng@lzb.ac.cn
  • 作者简介:顾燕玲(1971- ), 女, 甘肃张掖人, 1993年毕业于河西学院, 现为石河子大学在读硕士研究生, 主要从事冷环境微生物生态研究
  • 基金资助:

    国家自然科学基金项目(40961002; 41140009; 41271268); 冻土工程国家重点实验室开放基金项目(SKLFSE200903); 中国博士后科学基金特别资助项目(20100339)资助

Vertical Distribution Pattern of the Archaea Community Within the Permafrost Active Layer in Front of the Glacier No. 1 at Headwaters of Ürümqi River, Tianshan Mountains

GU Yan-ling1, SHI Xue-wei2, ZHU Jian-bo1, NI Yong-qing2,3, CHENG Guo-dong3   

  1. 1. School of Life Sciences, Shihezi University, Shihezi Xinjiang 832000, China;
    2. School of Food Sciences, Shihezi University, Shihezi Xinjiang 832000, China;
    3. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou Gansu 730000, China
  • Received:2013-01-13 Revised:2013-05-05 Online:2013-07-25 Published:2013-07-25

摘要:

采用免培养的PCR-DGGE(变性梯度凝胶电泳)指纹、 克隆测序、 系统发育分析对天山乌鲁木齐河源1号冰川前沿高山草甸冻土活动层古菌群落的垂直分格局进行了研究.结果表明: 1号冰川前沿高山草甸冻土活动层存在一些优势种群, 在所有6个取样深度的土层中均有分布.但不同深度土层古菌群落结构存在明显的差异, 在浅表层检测到一些特异的序列, 系统发育分析表明: 它们分别隶属于广古菌门(Euryarchaeota)的盐杆菌纲(Halobacteria)和热原体纲(Thermoplasmata), 而在深层土样没有检测到. 1号冰川前沿高山草甸带冻土活动层古菌群落以奇古菌(Thaumarchaeota)类群占据绝对优势, 全部隶属于被称为group1.1b的谱系.其中, 一些序列与不可培养的Nitrososophaera氨氧化古菌序列亲缘关系较近, 可能预示着这些中温泉古菌在氨氧化过程中发挥着重要作用, 对其群落结构及生态学贡献还有待更深入研究.

关键词: 乌鲁木齐河源1号冰川, 冻土活动层, 古菌群落, 垂直分布, PCR-DGGE

Abstract:

The diversity and vertical distribution of archaea community within the permafrost active layer in the proglacial environments of the Glacier No. 1, Tianshan Mountains are described by PCR-DGGE fingerprinting in the present study. Of the total 34 DGGE bands selected for sequencing, none of the sequences are the same as reference sequences from cultured taxa. 29 are identified as representing of non-thermophilic Crenarchaeota (Thaumarchaeota), while the remaining five are assigned as representing of Euryarchaeota, and clustered with members of the Halobacteria and Thermoplasmata. No sequences from methanogen are observed. Particularly, members of Thaumarchaeota Group 1.1b are present within the permafrost active layer in the proglacial environments of the Glacier No. 1, Tianshan Mountains (though lacking the defined structure) and Euryarchaeota communities are present only in the upper 10~100 cm of the layer, suggesting the relative abundance of Euryarchaeota decreasing with depth. In addition, some of phylotypes, which belong to Thaumarchaeota Group 1.1b, are closely related to ammonia-oxidizing Nitrososophaera. These results suggest that Thaumarchaeota Group 1.1b are active contributors to the N cycle throughout the permafrost active layer in the proglacial environments of the glacier, of which the contribution to community structure and ecology are necessary to study in the future in this area.

Key words: Glacier No. 1, Tianshan Mountains, permafrost active layer, archaea community, vertical distribution, PCR-DGGE

中图分类号: 

  • Q938