祁连山疏勒河上游芨芨草根际可培养细菌群落特征研究

doi:10.7522/j.issn.1000-0240.2014.0028

冰川冻土 ›› 2014, Vol. 36 ›› Issue (1): 222-229.doi: 10.7522/j.issn.1000-0240.2014.0028

祁连山疏勒河上游芨芨草根际可培养细菌群落特征研究

杨秀丽¹, 张宝贵², 张威², 刘光琇², 陈拓², 薛林贵¹

1. 兰州交通大学化学与生物工程学院, 甘肃兰州 730070;
2. 中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃兰州 730000

收稿日期:2013-09-07 修回日期:2013-12-29 出版日期:2014-02-25 发布日期:2014-03-18
通讯作者: 薛林贵，E-mail：Xuelg@mail.lzjtu.cn E-mail:Xuelg@mail.lzjtu.cn
作者简介:杨秀丽（1987-），女，甘肃金昌人，2011年毕业于天水师范学院，现为兰州交通大学在读硕士研究生，主要从事环境微生物研究
基金资助:
国家重点基础研究发展计划（973计划）项目（2012CB026105）；国家自然科学基金项目（30800154；31170465；31100365）资助

Characteristics of cultivable bacterial community in rhizosphere soil of Achnatherum splendens Trin in the upper reaches of the Shule River, Qilian Mountains

YANG Xiuli¹, ZHANG Baogui², ZHANG Wei², LIU Guangxiu², CHEN Tuo², XUE Lingui¹

1. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China

Received:2013-09-07 Revised:2013-12-29 Online:2014-02-25 Published:2014-03-18

摘要/Abstract

摘要：

以疏勒河上游不同海拔芨芨草根际土壤样品为研究对象，研究了不同海拔土样中细菌分布特征及其影响因素. 结果表明：研究区域芨芨草根际土壤可培养细菌种群密度变化范围为1.7×10⁷~10.8×10⁷CFU·g^-1，平均值为6.4×10⁷ CFU·g^-1，随海拔的升高呈先下降后上升的趋势；可培养细菌数量与土壤全氮、脲酶、蔗糖酶含量呈极显著正相关关系，与有机碳、磷酸酶含量呈显著正相关关系；同时，pH值也是影响细菌数量与多样性的一个重要因素. 通过16S rDNA基因测序及构建系统发育树，研究区域可培养细菌归类为15个属，其中芽孢杆菌属和假单胞菌属为优势菌属.

关键词: 疏勒河上游, 芨芨草, 根际细菌, 土壤理化性质, 系统发育树

Abstract:

In this study, the bacterial distribution and diversity in rhizosphere soil of Achnatherum splendens Trin changing with altitude in the upper reaches of Shule River were studied. The study results show that the number of cultivable bacteria varied between 1.7×10⁷-10.8×10⁷CFU·g^-1, with an average of 6.4 ×10⁷CFU·g^-1; there was a tendency that the number of cultivable bacteria decreased first and then increased; there was a significantly positive correlation between the number of cultivable bacteria and soil total nitrogen, urease and sucrose; the relationship between the number of cultivable bacteria and organic carbon and phosphatase was positive too; pH was a negative factor, which influences the number of cultivable bacteria and the diversity of bacteria. Based on 16S rDNA gene sequences and the phylogenetic tree, the cultivable bacteria in the study area include 15 genera, of which Pseudomonas and Bacillus genus are the dominate bacteria.

Key words: upper reaches of the Shule River, Achnatherum splendens Trin, rhizosphere bacteria, soil physic-chemical properties, phylogenetic tree

中图分类号:

Q938.1

杨秀丽, 张宝贵, 张威, 刘光琇, 陈拓, 薛林贵. 祁连山疏勒河上游芨芨草根际可培养细菌群落特征研究[J]. 冰川冻土, 2014, 36(1): 222-229.

YANG Xiuli, ZHANG Baogui, ZHANG Wei, LIU Guangxiu, CHEN Tuo, XUE Lingui. Characteristics of cultivable bacterial community in rhizosphere soil of Achnatherum splendens Trin in the upper reaches of the Shule River, Qilian Mountains[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2014, 36(1): 222-229.

参考文献

[1] Raaijmakers J M, Paulitz T C, Steinberg C, et al. The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms[J]. Plant and Soil, 2009, 321(1/2): 341-361.
[2] Walker T S, Bais H P, Halligan K M, et al. Metabolic profiling of root exudates of Arabidopsis thaliana[J]. Journal of Agriculture Food Chemistry, 2003, 51(9): 2548-2554.
[3] Conrad R. Soil microorganisms as controllers of atmospheric tra-ce gases (H₂, CO, CH₄, OCS, N₂O, and NO)[J]. Microbiology Review, 1996, 60(4): 609-640.
[4] Le Mer J, Roger P. Production, oxidation, emission and consumption of methane by soils: A review[J]. Europe Journal of Soil Biology, 2001, 37(1): 25-50.
[5] An Maozhu, Gao Wa, Wang Chunlan, et al. The natural and economic characteristics of Achnatherum splendens and its utilization and improvement[J]. Grassland of China, 2002, 24(5): 73-76. [安卯柱, 高娃, 王春兰, 等. 芨芨草的自然-经济特性及其利用与培育[J]. 中国草地, 2002, 24(5): 73-76.]
[6] Wang Zhongke, Yan Ping, Huang Gang, et al. Effect of artificial planting Achnatherum splendens on soil microbe quantity in desert[J]. Journal of Anhui Agriculture, 2007, 35(16): 4888-4889. [王仲科, 阎平, 黄刚, 等. 沙漠人工种植芨芨草对土壤微生物数量的影响[J]. 安徽农业科学, 2007, 35(16): 4888-4889.]
[7] Hu Wenge, Zhao Yadong, Yan Ping, et al. The preliminary analysis of soil microbial community of planting Achnatherum splendens (Trin) Nevski under saline and alkali land environment [J]. Ecology and Environment, 2007, 16(1): 197-200. [胡文革, 赵亚东, 闫平, 等. 盐碱地环境下芨芨草土壤微生物群落的初步分析[J]. 生态环境, 2007, 16(1): 197-200.]
[8] Ding Hongwei, Zhao Cheng, Huang Xiaohui. Ecological environment and desertification in Shulehe River basin[J]. Arid Zone Research, 2001, 18(2): 5-10. [丁宏伟, 赵成, 黄晓辉. 疏勒河流域的生态环境与沙漠化[J]. 干旱区研究, 2001, 18(2): 5-10.]
[9] Lin Gonghua, Yang Chuanhua, Chen Shengyun, et al. Large size soil animal communities of the frost soil regions in the upper reaches of Shule River[J]. Pratacultural Science, 2011, 28(10): 1864-1868. [林恭华, 杨传华, 陈生云, 等. 疏勒河上游冻土区大型土壤动物群落调查[J]. 草业科学, 2011, 28(10): 1864-1868.]
[10] Chen Shengyun, Liu Wenjie, Ye Baisheng, et al. Species diversity of vegetation in relation to biomass and environmental factors in the upper area of the Shule River[J]. Acta Prataculturae Sinica, 2011, 20(3): 71-83. [陈生云, 刘文杰, 叶柏生, 等. 疏勒河上游地区植被物种多样性和生物量及其与环境因子的关系[J]. 草业学报, 2011, 20(3): 71-83.]
[11] Sheng Yu, Li Jing, Wu Jichun, et al. Distribution patterns of permafrost in the upper area of Shule River with the application of GIS technique[J]. Journal of China University of Mining & Technology, 2010, 39(1): 32-39. [盛煜, 李静, 吴吉春, 等. 基于GIS的疏勒河流域上游多年冻土分布特征[J]. 中国矿业大学学报, 2010, 39(1): 32-39.]
[12] Sun Xike, Zhou Lihua, Chen Yong. The adaptive countermeasures against climate change in Shulehe River basin, China[J]. Journal of Desert Research, 2011, 31(5): 1316-1322. [孙希科, 周立华, 陈勇. 疏勒河流域气候变化情境下的适应对策[J]. 中国沙漠, 2011, 31(5): 1316-1322.]
[13] Zhang Gaosen, Zhang Wei, Liu Guangxiu, et al. Distribution of aerobic heterotrophic bacteria managed by environmental factors in glacier foreland[J]. Journal of Glaciology and Geocryology, 2012, 34(4): 965-971. [章高森, 张威, 刘光琇, 等. 环境因素主导着冰川前沿裸露地好氧异养细菌群落的分布[J]. 冰川冻土, 2012, 34(4): 965-971.]
[14] Zhang Baogui, Zhang Wei, Liu Guangxiu, et al. Effect of freeze-thaw cycles on the soil bacterial communities in different ecosystem soils in the Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2012, 34(6): 1499-1507. [张宝贵, 张威, 刘光琇, 等. 冻融循环对青藏高原腹地不同生态系统土壤细菌群落结构的影响[J]. 冰川冻土, 2012, 34(6): 1499-1507.]
[15] Guan Songyin. Soil Enzyme and Its Study Method[M]. Beijing: China Agriculture Press, 1986: 7. [关松荫. 土壤酶及其研究法[M]. 北京: 中国农业出版社, 1986: 7.]
[16] Zhang Gaosen, Niu Fujun, Ma Xiaojun, et al. Phylogenetic diversity of bacteria isolated from the Qinghai-Tibet Plateau permafrost region[J]. Canadian Journal of Microbiology, 2007, 53(8): 1000-1010.
[17] Wendu Rile, Zhang Jingni, Li Gang, et al. Effect of grazing disturbance on soil microorganisms and soil enzyme activities of Stipa baicalensis Rosev. steppe[J]. Acta Agrestia Sinica, 2010, 18(4): 517-522. [文都日乐, 张静妮, 李刚, 等. 放牧干扰对贝加尔针茅草原土壤微生物与土壤酶活性的影响[J]. 草地学报, 2010, 18(4): 517-522.]
[18] Mao Wenliang, Tai Xisheng, Wu Xiukun, et al. Altitudinal variation chatacteristics of the cultivable soil bacterial community on th upper reaches of the Heihe River, Qilian Mountains[J]. Journal of Glaciology and Geocryology, 2013, 35(2): 447-456. [毛文梁, 台喜生, 伍修琨, 等. 黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征[J]. 冰川冻土, 2013, 35(2): 447-456.]
[19] Dong Kang, Li Shiweng, Kang Wenlong, et al. Study of the changes in microbe amount and its affect factors in the soils along the Qinghai-Tibet Highway[J]. Journal of Glaciology and Geocryology, 2013, 35(2): 457-464. [董康, 李师翁, 康文龙, 等. 青藏公路沿线土壤微生物数量变化及其影响因素研究[J]. 冰川冻土, 2013, 35(2): 457-464.]
[20] Vega N W O. A review on beneficial effects of rhizosphere bacteria nutrient availability and plant nutrient uptake[J]. Revista Facultad Nacional de Agronomía-Medellín, 2007, 60(1): 3621-3643.
[21] Brown M E. Seed and root bacterization[J]. Annual Review Phytopathology, 1974, 12: 181-197.
[22] Sun Haixin, Liu Xunli. Microbes studies of tea rhizosphere[J]. Acta Ecologica Sinica, 2004, 24(7): 1353-1357. [孙海新, 刘训理. 茶树根际微生物研究[J]. 生态学报, 2004, 24(7): 1353-1357.]
[23] Stéphane U, Marc B, Claude M, et al. Pyrosequencing reveals a contrasted bacterial diversity between oak rhizosphere and surrounding soil[J]. Environmental Microbiology Reports, 2010, 2(2): 281-288.
[24] Benizri E, Piutti E, Verger S, et al. Replant diseases: Bacterial community structure and diversity in peach rhizosphere as determined by metabolic and genetic fingerprinting[J]. Soil Biology and Biochemistry, 2005, 37(9): 1738-1746.
[25] Marcela A F, Eugenia M, Mónica A L, et al. Molecular characterization and in situ detection of bacterial communities associated with rhizosphere soil of high altitude native Poaceae from the Andean Puna region[J]. Journal of Arid Environments, 2010, 74(10): 1177-1185.
[26] Rosa M, Jack W F. Mrakiella cryoconiti gen. nov., sp. nov., a psychrophilic, anamorphic, basidiomycetous yeast from alpine and arctic habitats[J]. International Journal of Systematic and Evolutionary Microbiology, 2008, 58(12): 2977-2982.
[27] Faoro H, Alves A C, Souza E M, et al. Influence of soil characteristics on the diversity of bacteria in the southern Brazilian Atlantic Forest[J]. Applied and Environmental Microbiology, 2010, 7(14): 4744-4749.
[28] Staddon T, Thompson K, Jakobsen S, et al. Mycorrhizal fungal abundance is affected by long-term climatic manipulation in the field[J]. Global Change Biology, 2003, 9(2): 186-194.
[29] Wang Meng, Chen Jiakuan, Li Bo. Characterization of bacterial community structure and diversity in rhizosphere soils of three plants in rapidly changing salt marshes using 16S rDNA[J]. Pedosphere, 2007, 17(5): 545-556.
[30] Gundapally S R, Garcia-Pichel F. The community and phylogenetic diversity of biological soil crusts in the Colorado Plateau studied by molecular fingerprinting and intensive cultivation[J]. Microbial Ecology, 2006, 52(2): 354-357.
[31] Hu Ping, Wu Xiukun, Li Shiweng, et al. Progress of studies on permafrost microbial ecology in the past 10 years[J]. Journal of Glaciology and Geocryology, 2012, 34(3): 732-739. [胡平, 伍修琨, 李师翁, 等. 近10 a来冻土微生物生态学研究进展[J]. 冰川冻土, 2012, 34(3): 732-739.]
[32] Liu Guangxiu, Hu Ping, Zhang Wei, et al. Variations in soil culturable bacteria communities and biochemical characteristics in the Dongkemadi glacier forefield along a chronosequence[J]. Folia Microbiology, 2012, 57(6): 485-494.

祁连山疏勒河上游芨芨草根际可培养细菌群落特征研究

Characteristics of cultivable bacterial community in rhizosphere soil of Achnatherum splendens Trin in the upper reaches of the Shule River, Qilian Mountains

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