X img

官方微信

img

群号:冰川冻土交流群

QQ群:218834310

高级检索

冰川冻土 ›› 2014, Vol. 36 ›› Issue (2): 457-461.doi: 10.7522/j.issn.1000-0240.2014.0055

• 寒旱区生物学 • 上一篇    下一篇

青海湖裸鲤内脏发光细菌的分离和鉴定

卓平清1,2, 米琴3, 李三相2, 薛林贵1, 王弋博1,2   

  1. 1. 兰州交通大学 化学与生物工程学院, 甘肃 兰州 730070;
    2. 天水师范学院 生命科学与化学学院, 甘肃 天水 741000;
    3. 青海师范大学 生命与地理科学学院, 青海 西宁 810008
  • 收稿日期:2013-09-03 修回日期:2013-12-27 出版日期:2014-04-25 发布日期:2014-05-20
  • 作者简介:卓平清(1985-),女,广西梧州人,2011年毕业于青海师范大学,现为兰州交通大学硕士研究生,主要从事环境与资源微生物研究. E-mail:pqzhuo666@foxmail.com
  • 基金资助:
    国家自然科学基金项目(31060065;41161058);甘肃省自然科学基金项目(1107RJZE117)资助

Isolation and identification of the luminous bacteria from the gut of Gymnocypris przewalskii

ZHUO Pingqing1,2, MI Qin3, LI Sanxiang2, XUE Lingui1, WANG Yibo1,2   

  1. 1. School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
    2. School of Life Science and Chemistry, Tianshui Normal University, Tianshui 741000, Gansu, China;
    3. School of Life Science and Geographical Science, Qinghai Normal University, Xining 810008, China
  • Received:2013-09-03 Revised:2013-12-27 Online:2014-04-25 Published:2014-05-20
  • Contact: 王弋博,E-mail:wangyb02@126.com E-mail:wangyb02@126.com

摘要: 2009-2013年,从青海湖裸鲤内脏器官中共分离获得6株发光细菌,编号依次为Q1、Q2、Q3、Q4、Q5、Q6. 这6株发光细菌细胞壁革兰氏染色均为阴性,细胞为杆状,大小为(0.5~0.8) μm×(1.0~1.5) μm,具有单极生鞭毛,有运动性. 氧化酶和接触酶均呈阳性反应,能发酵葡萄糖产酸;吲哚试验和V-P试验均为阳性反应,能分泌淀粉酶、明胶酶、DNA酶,能将硝酸盐还原为亚硝酸盐. 最适生长温度为20~25 ℃,最适生长pH为8.5~9.0. 16S rDNA基因序列分析结果表明,6株发光菌与其亲缘关系最近的属为Vibrio(弧菌属),该属内和此次实验的6株发光菌亲缘关系最近的种Vibrio anguillarum(鳗弧菌)的16S rDNA序列相似值为99.57%. Biolog Gen Ⅲ鉴定结果显示,该实验的6株发光菌在有氧条件下,在提供的71种碳源中,只能利用其中的30种,其中,糖类12种,氨基酸类5种,羧酸类4种,聚合物类2种,其他类7种. 结合6株发光菌的形态学特征、生理生化特性、Biolog Gen Ⅲ鉴定结果和分子生物学特性,将菌株鉴定为Vibrio anguillarum. 该菌所具有的发光特性为首次报道.

关键词: 青海湖裸鲤, 内脏, 发光细菌, 鉴定, 青海湖

Abstract: Six luminous bacteria, numbered Q1, Q2, Q3, Q4 ,Q5 and Q6, were isolated from the gut of Gymnocypris przewalskii from 2009 to 2013. Strains Q1~Q6 are bacteria of gram-negative, rod shaped ((0.5~0.8)μm×(1.0~1.5)μm), polar flagella, motile, oxidase, catalase, nitrate reduction test, indole and V-P test-positive. Strains Q1~Q6 are able to ferment glucose and produce acid, secret amylase, gelatinase and DNase. The optimum growth temperature is 20~25 ℃ and the optimum growth pH value is 8.5~9.0. Analysis of 16S rDNA of Q1~Q6 indicates that the isolate is able to form a monophyletic clade with the members of the genus Vibrio. The closest phylogenetic relative among the valid species is Vibrio anguillarum, with 99.57% similarity. Tested by using Biology GENⅢ system, the strains could utilize 30 carbohydrates (12 saccharides, 5 amino acids, 4 carboxylic acids, 2 polymers and 7 others) of 71 carbohydrates under aerobic growth condition. The isolates are identified as strains of Vibrio anguillarum based on their biochemical, physiological, the results of Biology GENⅢ system identification and morphological characteristics, as well as the analysis of 16S rDNA sequence. Characteristics of luminescence of Vibrio anguillarum reported here are new finding.

Key words: Gymnocypris przewalskii, gut, luminous bacteria, identification, Qinghai Lake

中图分类号: 

  • Q939