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冰川冻土 ›› 2016, Vol. 38 ›› Issue (3): 785-793.doi: 10.7522/j.issn.1000-0240.2016.0088

• 寒旱区生物学 • 上一篇    下一篇

祁连山不同海拔低温原油降解菌群的分布特性研究

杨蕊琪1, 薛林贵1, 常思静2, 章高森2, 陈拓2, 刘光琇2, 张勇3, 高海宁3   

  1. 1. 兰州交通大学 化学与生物工程学院 甘肃省极端环境微生物资源与工程重点实验室, 甘肃 兰州 730070;
    2. 甘肃省极端环境微生物资源与工程重点实验室/中国科学院 寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃 兰州 730030;
    3. 河西学院 农业与生物技术学院, 甘肃 张掖 734000
  • 收稿日期:2016-02-13 修回日期:2016-05-04 出版日期:2016-06-25 发布日期:2016-09-21
  • 通讯作者: 薛林贵,E-mail:xuelg@mail.lzjtu.cn. E-mail:xuelg@mail.lzjtu.cn
  • 作者简介:杨蕊琪(1989-),女,甘肃平凉人,2013年毕业于陇东学院,现为兰州交通大学在读硕士研究生,主要从事环境微生物学方面的研究.E-mail:514503456@qq.com
  • 基金资助:
    国家国际科技合作专项项目(2014DFA30330);甘肃省国际科技合作项目(1304WCGA173);国家自然科学基金项目(31260090)资助

Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains

YANG Ruiqi1, XUE Lingui1, CHANG Sijing2, ZHANG Gaosen2, CHENG Tuo2, LIU Guangxiu2, ZHANG Yong3, GAO Haining3   

  1. 1. College of Chemical and Biological Engineering, Lanzhou Jiaotong University, Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou 730070, China;
    2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730030, China;
    3. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, Gansu, China
  • Received:2016-02-13 Revised:2016-05-04 Online:2016-06-25 Published:2016-09-21

摘要: 以祁连山南麓中端野牛沟土壤为研究对象,研究了不同海拔下低温原油降解细菌群落的分布特征。结果显示:研究区域具有原油利用潜力的低温降解菌数量为0.3×105~1.4×105CFU ·g-1,随海拔的升高,菌群明显减少;同时通过16S rRNA基因序列分析共发现4个门、7个属、25种细菌,其中假单胞菌属(Pseudomonas)和节杆菌属(Arthrobacter)是优势属,并且位于海拔3597m处的菌株原油利用潜力均较高,而变形菌门的不动杆菌属(Acinetobacter)利用原油生长的潜力最高。

关键词: 祁连山, 海拔, 低温, 原油降解菌

Abstract: In this study,the distribution characteristics of low-temperature crude oil degrading bacterial population in soil from Yeniugou in the south side of the Qilian Mountains was investigated. The results showed that the quantities of degrading bacterium in this region were between 0.3×105~1.4×105CFU·g-1,which also decreased with altitude. Meanwhile,through 16S rRNA gene sequence analysis,it was detected that there were four phylum,seven genera and 25 species;among them,Pseudomonas and Arthrobacter were predominant genera. It was also found that the strains isolated from the altitude of 3579m had relatively higher utilization potential and the strains belonging to the genus Acinetobacter had the highest utilization potential for crude-oil.

Key words: Qilian Mountains, altitude, low-temperature, crude oil degrading bacterium

中图分类号: 

  • Q938.1