祁连山不同海拔低温原油降解菌群的分布特性研究

doi:10.7522/j.issn.1000-0240.2016.0088

冰川冻土 ›› 2016, Vol. 38 ›› Issue (3): 785-793.doi: 10.7522/j.issn.1000-0240.2016.0088

祁连山不同海拔低温原油降解菌群的分布特性研究

杨蕊琪¹, 薛林贵¹, 常思静², 章高森², 陈拓², 刘光琇², 张勇³, 高海宁³

1. 兰州交通大学化学与生物工程学院甘肃省极端环境微生物资源与工程重点实验室, 甘肃兰州 730070;
2. 甘肃省极端环境微生物资源与工程重点实验室/中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃兰州 730030;
3. 河西学院农业与生物技术学院, 甘肃张掖 734000

收稿日期:2016-02-13 修回日期:2016-05-04 出版日期:2016-06-25 发布日期:2016-09-21
通讯作者: 薛林贵,E-mail:xuelg@mail.lzjtu.cn. E-mail:xuelg@mail.lzjtu.cn
作者简介:杨蕊琪(1989-),女,甘肃平凉人,2013年毕业于陇东学院,现为兰州交通大学在读硕士研究生,主要从事环境微生物学方面的研究.E-mail:514503456@qq.com
基金资助:
国家国际科技合作专项项目（2014DFA30330）；甘肃省国际科技合作项目（1304WCGA173）；国家自然科学基金项目（31260090）资助

Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains

YANG Ruiqi¹, XUE Lingui¹, CHANG Sijing², ZHANG Gaosen², CHENG Tuo², LIU Guangxiu², ZHANG Yong³, GAO Haining³

1. College of Chemical and Biological Engineering, Lanzhou Jiaotong University, Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Lanzhou 730070, China;
2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730030, China;
3. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, Gansu, China

Received:2016-02-13 Revised:2016-05-04 Online:2016-06-25 Published:2016-09-21

摘要/Abstract

摘要： 以祁连山南麓中端野牛沟土壤为研究对象，研究了不同海拔下低温原油降解细菌群落的分布特征。结果显示：研究区域具有原油利用潜力的低温降解菌数量为0.3×10⁵~1.4×10⁵CFU ·g^-1，随海拔的升高，菌群明显减少；同时通过16S rRNA基因序列分析共发现4个门、7个属、25种细菌，其中假单胞菌属（Pseudomonas）和节杆菌属（Arthrobacter）是优势属，并且位于海拔3597m处的菌株原油利用潜力均较高，而变形菌门的不动杆菌属（Acinetobacter）利用原油生长的潜力最高。

关键词: 祁连山, 海拔, 低温, 原油降解菌

Abstract: In this study,the distribution characteristics of low-temperature crude oil degrading bacterial population in soil from Yeniugou in the south side of the Qilian Mountains was investigated. The results showed that the quantities of degrading bacterium in this region were between 0.3×10⁵~1.4×10⁵CFU·g^-1,which also decreased with altitude. Meanwhile,through 16S rRNA gene sequence analysis,it was detected that there were four phylum,seven genera and 25 species;among them,Pseudomonas and Arthrobacter were predominant genera. It was also found that the strains isolated from the altitude of 3579m had relatively higher utilization potential and the strains belonging to the genus Acinetobacter had the highest utilization potential for crude-oil.

Key words: Qilian Mountains, altitude, low-temperature, crude oil degrading bacterium

中图分类号:

Q938.1

杨蕊琪, 薛林贵, 常思静, 章高森, 陈拓, 刘光琇, 张勇, 高海宁. 祁连山不同海拔低温原油降解菌群的分布特性研究[J]. 冰川冻土, 2016, 38(3): 785-793.

YANG Ruiqi, XUE Lingui, CHANG Sijing, ZHANG Gaosen, CHENG Tuo, LIU Guangxiu, ZHANG Yong, GAO Haining. Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2016, 38(3): 785-793.

参考文献

[1] Head I M, Jones D M, Röling W F M. Marine microorganisms make a meal of oil[J]. Nature Reviews Microbiology, 2006,4(3):173-182.
[2] Jin Huijun,Yu Wenbing,Chen Youchang, et al. (Differential) frost heave and thaw settlement in the engineering design and construction of oil pipelines in permafrost regions:A review[J]. Journal of Glaciology and Geocryology, 2005, 27 (3):454-464. [金会军,喻文兵,陈友昌,等. 多年冻土区输油管道工程中的(差异性)融沉和冻胀问题[J]. 冰川冻土,2005,27(4):454-464.]
[3] Wang Yilin,Ai Xue,Li Shiweng,et al. Isolation,identification and degradation characteristics of a low temperature crude oil degrading bacteriastrain from the soil of Tibetan Plateau[J]. Journal of Glaciology and Geocryology,2015,37(2):528-537. [王艺霖,艾雪,李师翁,等. 青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性[J]. 冰川冻土, 2015,37(2):528-537.]
[4] Zhao Yuxia,Yang Ke. Review on remediation technology of petroleum- contaminated soil[J]. Environmental Science and Technology, 2009,22(S1):60-63.
[5] Wang Shijie,Wang Xiang,Lu Guilan,et al. Bioremediation of petroleum hydrocarbon- contaminated soils by cold- adapted microorganisms:Research advance[J]. Chinese Journal of Applied Ecology, 2011, 22(4): 1082-1088. [王世杰, 王翔,卢桂兰,等. 低温微生物修复石油烃类污染土壤研究进展[J]. 应用生态学报,2011,22(4):1082-1088.]
[6] Margesin R,Labbé D,Schinner F,et al. Characterization of hydrocarbon- degrading microbial populations in contaminated and pristine alpine soils[J]. Applied and Environmental Microbiology, 2003,69(6):3085-3092.
[7] Esmaeil ALS,Drobiova H,Obuekwe C. Predominant culturable crude oil-degrading bacteria in the coast of Kuwait[J]. International Biodeterioration & Biodegradation,2008,63(4): 400-406.
[8] Aislabie J,Saul D J,Foght J M. Bioremediation of hydrocarbon- contaminated polar soils[J]. Extremophiles, 2006, 10 (3):171-179.
[9] Wen Xi, Zhao Liang, Shi Yulan, et al. Significant shifts of bacterial communities in the active layer of permafrost caused by crude oil contamination:A case study from Jiagedaqi[J]. Journal of Glaciology and Geocryology, 2014, 36(5): 1306-1312. [文茜,赵亮,石玉兰,等. 短期原油污染致使冻土活动层细菌群落显著改变——以加格达奇冻土活动层为例[J]. 冰川冻土,2014,36(5):1306-1312.]
[10] Zhu Ping,Chen Rensheng,Song Yaoxuan,et al. Soil microbial community diversity under four vegetation types in the Qilian Mountains[J]. Acta Prataculturae Sinica, 2015, 24(6): 75-84. [朱平,陈仁升,宋耀选,等. 祁连山不同植被类型土壤微生物群落多样性差异[J]. 草业学报,2015,24(6): 75-84.]
[11] Yang Quansheng,Wang Youkui, Li Jinjun, et al. Effective analysis on natural forest protection project in National Nature Reserve of Qilian Mountains[J]. Journal of Central South University of Forestry & Technology,2015,35(1):89-95.[杨全生,汪有奎,李进军,等. 祁连山自然保护区天然林保护工程的成效分析[J]. 中南林业科技大学学报,2015,35 (1):89-95.]
[12] Jia Yan,Yin Hua,Peng Hui,et al. Isolation,Identification and characteristics of crude oil- degrading microorganism[J]. Journal of Jinan University(Natural Science),2007,28(3): 296-301. [贾燕,尹华,彭辉,等. 石油降解菌株的筛选、初步鉴定及其特性[J]. 暨南大学学报(自然科学版),2007, 28(3):296-301.]
[13] Wang Wanpeng,Wang Liping, Shao Zongze. Diversity and abundance of oil- degrading bacteria and alkane hydroxylase (alkB)genes in the subtropical seawater of Xiamen island[J]. Microbial Ecology,2010,60(2):429-439.
[14]Wang Baojiang,Lai Qiliang,Cui Zhisong,et al. A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1[J]. Environmental Microbiology,2008,10(8):1948-1963.
[15] Yang Xiuli,Zhang Baogui,Zhang Wei,et al. Characteristics of cultivable bacterial community in rhizosphere soil of Achnatherum splendens Trin in the upper reaches of the Shule Riv-er,Qilian Mountains[J]. Journal of Glaciology and Geocryology, 2014,36(1):222-229. [杨秀丽,张宝贵,张威, 等. 祁连山疏勒河上游芨芨草根际可培养细菌群落特征研究[J]. 冰川冻土,2014,36(1):222-229.]
[16] Qian P,Schoenaru J J,Karamanos R E. Simultaneous extraction of available phosphorus and potassium with a new soil test: A modification of Kelowna extraction[J]. Communications in Soil Science & Plant Analysis,1994,25(5/6):627-635.
[17] Dunbar J, Takala S, Barns S M, et al. Levels of bacterial community diversity in four arid soils compared by cultivation and 16S rRNA gene cloning[J]. Applied and environmental microbiology, 1999,65(4):1662-1669.
[18] Lane D J. 16S/23S rRNA sequencing[J]. Nucleic Acid Techniques in Bacterial Systematics,1991:125-175.
[19] Chen Wei,Zhang Wei,Li Shiweng,et al. Feature of soil cultivable microorganism quantity and diversity distribution under different alpine grassland ecosystems in Qinghai- Tibet Plateau[J]. Journal of Glaciology and Geocryology, 2011, 33(6): 1419-1426. [陈伟,张威,李师翁,等. 青藏高原不同类型草地生态系统下土壤可培养细菌数量及多样性分布特征研究[J]. 冰川冻土,2011,33(6):1419-1426.]
[20] Mao Wenliang, Tai Xisheng,Wu Xiukun, et al. Altitudinal variation characteristics of the cultivable soil bacterial community on the upper reaches of the Heihe River, Qilian Mountains[J]. Journal of Glaciology and Geocryology, 2013, 35(2): 447-456. [毛文梁,台喜生,伍修锟,等. 黑河上游祁连山区土壤可培养细菌群落生境的垂直分异特征[J]. 冰川冻土, 2013,35(2):447-456.]
[21] Tai Xisheng,Yang Xiuli,Liu Guangxiu,et al. Variations of N and P cycling bacterial populations along with altitude in the Qilian Mountains[J]. Journal of Glaciology and Geocryology, 2014,36(1):214-221. [台喜生,杨秀丽,刘光琇,等. 祁连山不同海拔氮磷循环细菌数量变化特征[J]. 冰川冻土, 2014,36(1):214-221.]
[22] Liu Bingru,Zhang Xiuzheng,Hu Tianhua,et al. Soil microbial diversity under typical vegetation zones along an elevation gradient in Helan Mountains[J]. Acta Ecologica Sinica,2013, 33(22):7211-7220. [刘秉儒,张秀珍,胡天华,等. 贺兰山不同海拔典型植被带土壤微生物多样性[J]. 生态学报, 2013,33(22):7211-7220.]
[23] Zhang Baogui, Zhang Wei, Liu Guangxiu, et al. Effect of Freeze-Thaw cycles on the soil bacterial communities in different ecosystem soils in the Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2012, 34(6): 1499-1507. [张宝贵,张威,刘光琇,等. 冻融循环对青藏高原腹地不同生态系统土壤细菌群落结构的影响[J]. 冰川冻土,2012,34 (6):1499-1507.]
[24] Panicker G,Mojib N,Aislabie J,et al. Detection,expression and quantitation of the biodegradative genes in Antarctic microorganisms using PCR [J]. Antonie van Leeuwenhoek, 2010,97(3):275-287.
[25] Ferrera- Rodríguez O, Greer C W, Juck D, et al. Hydrocarbon- degrading potential of microbial communities from Arctic plants[J]. Journal of Applied Microbiology, 2012, 114(1): 71-83.
[26] Li Li,Zhang Liping,Zhang Yuanliang. The research situation of petroleum hydrocarbons degradation bacteria[J]. Microbiology China,2001,28(5):89-92. [李丽,张利平,张元亮. 石油烃类化合物降解菌的研究概况[J]. 微生物学通报, 2001,28(5):89-92.]
[27] Zhu Shengfeng,Liang Shengkang,Wu Liang,et al. Effect of adding rhamnolipid biosurfactant and inoculating rhamnolipidproducing strain on oil biodegradation[J]. Environmental Science & Technology,2010,33(5):15-20. [朱生凤,梁生康,吴亮,等. 鼠李糖脂及其产生菌对原油生物降解影响研究[J]. 环境科学与技术,2010,33(5):15-20.]
[28] Wang Dongmei, Chen Lihua, Zhou Lihui, et al. Effects of rhamnolipid on petroleum degradation of compound microbial inoculant[J]. Chinese Journal of Environmental Engineering, 2013,7(10):4121-4126. [王冬梅,陈丽华,周立辉, 等. 鼠李糖脂对微生物菌剂降解石油的影响[J]. 环境工程学报,2013,7(10):4121-4126.]
[29] Maripat Turdahun,Abduwaly Abdurehim,Zilaygul Mijit,et al. Diversity of culturable bacteria isolated from two altitudes in the West Kunlun Mountains[J]. Arid Zone Research, 2011, 28(6):1071-1080. [玛丽帕·吐达洪,阿不都外力·阿布都热依木,孜来古丽·米吉提,等. 西昆仑山2 个海拔位点可培养细菌多样性分析[J]. 干旱区研究,2011,28(6): 1071-1080.]
[30] Li Zuyi. Properties and applications of biosurfactants[J]. Chinese Journal of Organic Chemistry,1986(3):177-183. [李祖义. 生物表面活性剂的特性及应用[J]. 有机化学,1986 (3):177-183.]
[31] Bragg J R, Prince R C, Harner E J, et al. Effectiveness of bioremediation for the Exxon Valdez oil spill[J]. Nature, 1994,368(6470):413-418.
[32] Kiesele L U. Efficient and cost-effective purification of groundwater polluted by polycyclic aromatic hydrocarbons experience with a coke-plant hazardous waste site[J]. Altlasten Spektrum, 1997,6(5):214-217.
[33] Cao Juan,Xu Zhihui, Li Lingzhi, et al. Biosurfactant producing petroleum degrader Acinetobacter BHSN[J]. Journal of Ecology and Rural Environment,2009,25(1):73-78. [曹娟,徐志辉,李凌之,等. 农村环境产生物表面活性剂的石油降解菌Acinetobacter BHSN 的研究[J]. 生态与学报, 2009,25(1):73-78.]
[34] Su Ying,Chen Li,Liu Zhaopu. Research on characteristics of a marine oil- degrading strain[J]. Research of Environmental Sciences,2008,21(5):32-36. [苏莹,陈莉,刘兆普. 一株海洋石油降解菌的特性研究[J]. 环境科学研究,2008,21 (5):32-36.]
[35] Sun Jiquan,Xu Lian,Tang Yueqin,et al. Simultaneous degradation of phenol and n- hexadecane by Acinetobacter strains[J]. Bioresource technology,2012,123:664-668.

祁连山不同海拔低温原油降解菌群的分布特性研究

Elevation-distribution characteristics of the crude oil degrading bacterium in the Qilian Mountains

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	马素辉, 牟翠翠, 郭红, 张现凯, 栗泽苑, 张廷军. 祁连山黑河上游多年冻土区不同植被类型土壤有机碳密度分布特征[J]. 冰川冻土, 2018, 40(3): 426-433.
[2]	韩涛, 王大为, 李丽丽. FY-3A/MERSI积雪制图中NDSI指标建立及积雪判识模型研究——以祁连山区为例[J]. 冰川冻土, 2018, 40(3): 511-527.
[3]	张欢, 平琦, 吴明静. 低温含水砂岩动态压缩力学性能试验研究[J]. 冰川冻土, 2018, 40(1): 79-85.
[4]	胡钰玲, 赵中军, 康彩燕, 康延臻, 王式功, 尚可政. 中国南方2008年与2016年两次低温雨雪冰冻天气过程对比分析[J]. 冰川冻土, 2017, 39(6): 1180-1191.
[5]	蒋友严, 杜文涛, 黄进, 赵慧珍, 王成福. 2000-2015年祁连山植被变化分析[J]. 冰川冻土, 2017, 39(5): 1130-1136.
[6]	王涛, 高峰, 王宝, 王鹏龙, 王勤花, 宋华龙, 尹常亮. 祁连山生态保护与修复的现状问题与建议[J]. 冰川冻土, 2017, 39(2): 229-234.
[7]	冯芳, 冯起, 刘贤德, 刘蔚, 金爽. 祁连山中段排露沟流域水化学特征及成因[J]. 冰川冻土, 2017, 39(2): 407-415.
[8]	杜玉霞, 马巍, 赵淑萍, 张泽, 明姣. 负温条件下激光位移传感器的标定[J]. 冰川冻土, 2016, 38(6): 1583-1591.
[9]	刘澄静, 角媛梅, 张贵玲, 刘歆, 章天应. 哈尼梯田麻栗寨河流域泉水氢氧同位素的海拔效应[J]. 冰川冻土, 2016, 38(5): 1404-1410.
[10]	贺建桥, 张伟, 周剑, 吴玉伟. 山脉走向对降水中δ¹⁸O垂直递减率的影响——以黑河流域上游祁连山区为例[J]. 冰川冻土, 2016, 38(2): 359-367.
[11]	杨志娟, 陈银萍, 李冬花, 张正庆, 郝海艳, 张风霞, 苏向楠. 外源脱落酸对低温胁迫下圆柏属植物幼苗生理特性的影响[J]. 冰川冻土, 2015, 37(6): 1642-1649.
[12]	牛赟, 刘贤德, 苗毓鑫, 车宗玺. 祁连山大野口流域土壤水热空间变化特征研究[J]. 冰川冻土, 2015, 37(5): 1353-1360.
[13]	吴家章, 易朝路, 许向科, 乔宝晋, 刘宇硕. 祁连山摆浪河全新世冰量变化初探[J]. 冰川冻土, 2015, 37(3): 595-603.
[14]	王波, 陈拓, 徐国保, 刘晓宏, 王文志, 吴国菊. 祁连山中部祁连圆柏林线树木生长与积雪响应关系研究[J]. 冰川冻土, 2015, 37(2): 318-326.
[15]	王艺霖, 艾雪, 李师翁, 龙昊知, 章高森, 张威, 刘光琇, 陈拓. 青藏高原土壤中一株低温原油降解菌的分离鉴定及其原油降解特性[J]. 冰川冻土, 2015, 37(2): 528-537.