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冰川冻土 ›› 2018, Vol. 40 ›› Issue (5): 1037-1046.doi: 10.7522/j.issn.1000-0240.2018.0309

• 寒旱区生物学 • 上一篇    下一篇

青藏高原土壤中一株原油降解菌的作用机制探究

常思静1,2,4, 杨蕊琪2,3,4, 章高森3,4, 刘光琇3,4, 陈拓1,4   

  1. 1. 中国科学院 西北生态环境资源研究院 冰冻圈科学国家重点实验室, 甘肃 兰州 730000;
    2. 中国科学院大学, 北京 100049;
    3. 中国科学院 西北生态环境资源研究院 沙漠与沙漠化重点实验室, 甘肃 兰州 730000;
    4. 中国科学院 西北生态环境资源研究院 甘肃省极端环境微生物资源与工程重点实验室, 甘肃 兰州 730000
  • 收稿日期:2017-12-01 修回日期:2018-02-16 出版日期:2018-10-25 发布日期:2018-12-10
  • 通讯作者: 陈拓,E-mail:chentuo@lzb.ac.cn. E-mail:chentuo@lzb.ac.cn
  • 作者简介:常思静(1983-),女,河北唐山人,2010年在兰州交通大学获硕士学位,现为中国科学院西北生态环境资源研究院在读博士研究生,从事微生物生态研究.E-mail:changsijinglanzhou@163.com
  • 基金资助:
    国家国际科技合作专项项目(2014DFA30330)资助

Investigation of crude oil-degrading mechanisms of a bacterial strain isolated from Tibetan Plateau soil

CHANG Sijing1,2,4, YANG Ruiqi2,3,4, ZHANG Gaosen3,4, LIU Guangxiu3,4, CHEN Tuo1,4   

  1. 1. State Key Laboratory of Cryospheric Sciences, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China;
    3. State Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China;
    4. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2017-12-01 Revised:2018-02-16 Online:2018-10-25 Published:2018-12-10

摘要: 分别采用原油和丙酮酸作为碳源培养Pedobacter steynii DX4细胞,采用Illumina高通量测序技术对两种碳源条件下细胞的转录组进行测序,测序数据拼接组装后共得到7 693个Unigene,其中有5 017个Unigene获得CDS注释。对Unigene进行筛选共获得1 195个差异表达基因,约占Unigene总数的15.5%,对差异表达基因进行功能分析发现,在原油为碳源的生长条件下,DX4细胞中Luciferase-like monooxygenase基因和卤代烷脱氢酶基因表达水平显著提高,这两个基因可能在DX4细胞中催化烷烃末端氧化反应。KEGG分析显示,以原油为碳源的DX4细胞中的脂肪酸降解途径中发现了8个UPDEGs、苯酸盐降解途径中发现了10个UPDEGs,揭示了细胞对原油中烷烃和芳香烃类物质的降解通路。此外,关于信号处理、物质转运、胞外多糖合成以及细胞趋向运动代谢途径的基因表达水平上调,表明DX4细胞可能依赖细胞趋向运动和合成生物表面活性物质来辅助原油降解。

关键词: 微生物, 原油降解, 代谢途径, 青藏高原

Abstract: Crude oil and pyruvate as carbon source were used respectively to cultivate Pedobacter steynii DX4 cells, using Illumina high-throughput sequencing technologies to the cells of two kinds of carbon sources under the condition of the transcriptome sequencing. Sequencing data were spliced and assembled into 7 693 unigene, among them there were 5 017 unigene identified as CDS annotation. Filtering the unigene had received 1 195 differentially expressed genes, accounts for about 15.5% of total unigene, of which functional analyses had found that under the condition of growth of the crude oil as carbon source, DX4 cells luciferase (like monooxygenase genes and halogenated dehydrogenase gene) expression level increased significantly, the two genes may catalyze the ends of alkane oxidation reaction in DX4 cells. The differently expressed genes were annotated according to the COG and KEGG databases. The cells, which fed on crude oil, expression levels of genes coding for luciferase-like monooxygenase and haloalkane dehalogenase were significantly up-regulated. The luciferase-like monooxygenase and haloalkane dehalogenase were deduced to be the key enzymes catalyzing alkane terminal oxidations in DX4. KEGG annotation showed that there were 8 UPDEGs in fatty acid degradation pathway and 10 UPDEGs in benzoate degradation pathway, indicating that the petroleum components were bio-degraded through these pathways. In addition, many genes were up-regulated in oil treated cells, including genes related to signal transduction, material transport, extracellular polysaccharide synthesis and chemotaxis. The chemotaxis and biosurfactant may be useful assisted mechanisms in DX4 when cells degrade crude oil.

Key words: Microbial, crude oil degradation, metabolic pathway, Tibetan Plateau

中图分类号: 

  • X172