青藏高原北麓河地区荒漠草原土壤细菌对热融滑塌的响应

doi:10.7522/j.issn.1000-0240.2019.0118

冰川冻土 ›› 2019, Vol. 41 ›› Issue (4): 977-985.doi: 10.7522/j.issn.1000-0240.2019.0118

青藏高原北麓河地区荒漠草原土壤细菌对热融滑塌的响应

张晓兰^1,2, 刘桂民¹, 李新星¹, 吴小丽¹, 徐海燕¹, 纪庚好¹, 李莉莎¹, 吴晓东²

1. 兰州交通大学环境与市政工程学院, 甘肃兰州 730070;
2. 中国科学院西北生态环境资源研究院冰冻圈科学国家重点实验室/青藏高原冰冻圈观测研究站, 甘肃兰州 730000

收稿日期:2018-08-06 修回日期:2019-01-07 出版日期:2019-08-25 发布日期:2019-11-28
通讯作者: 刘桂民,E-mail:liuguimin@mail.lzjtu.cn. E-mail:liuguimin@mail.lzjtu.cn
作者简介:张晓兰(1991-),女,甘肃皋兰人,2016年在兰州城市学院获学士学位,现为兰州交通大学在读硕士研究生,从事寒区生态学研究.E-mail:zhangxl369369@163.com
基金资助:
国家自然科学基金项目（41661013；41871060；41861011）资助

The response of soil bacteria to thaw slump in a desert steppe in Beiluhe area, Qinghai-Tibet Plateau

ZHANG Xiaolan^1,2, LIU Guimin¹, LI Xinxing¹, WU Xiaoli¹, XU Haiyan¹, JI Genghao¹, LI Lisha¹, WU Xiaodong²

1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Cryosphere Research Station on the Qinghai-Tibet Plateau, State Key Laboratory of Cryospheric Sciences, Northwest Institute of the EcoEnvironment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China

Received:2018-08-06 Revised:2019-01-07 Online:2019-08-25 Published:2019-11-28

摘要/Abstract

摘要： 多年冻土区地下冰融化造成的热融滑塌会对土壤理化性质产生一系列影响，进而影响微生物群落结构，但目前热融滑塌对荒漠草原区土壤微生物的影响还不清楚。利用Illumina测序方法，以青藏高原北麓河多年冻土区发生热融滑塌的荒漠草原为研究对象，对3种微地貌（对照区、滑塌区、沉降区）下的土壤细菌展开研究，分析了细菌群落结构及其与环境因子间的关系。结果显示滑塌区和沉降区有机碳含量显著低于对照区，滑塌区土壤含水量最高；放线菌门、变形菌门、绿弯菌门和酸杆菌门是3种微地貌下的优势菌群，对照区细菌群落丰富度显著低于滑塌区。Mantel检验表明，细菌群落结构与土壤理化性质间无显著相关性；非度量多维尺度分析（NMDS）表明，微生物群落结构在3种微地貌下显著不同。研究结果表明，在荒漠草原区，热融滑塌会改变土壤理化性质，影响细菌特定门的微生物相对丰度，但对门水平的整体群落结构影响不显著。

关键词: 热融滑塌, 青藏高原, 荒漠草原, 细菌群落, 多年冻土, 高通量测序

Abstract: Thaw slumping is caused by the melting of ground ice in permafrost,which may impact soil physical and chemical properties and thus affect the microbial community structures. However,the effect of thaw slump on the soil microbes under desert steppe remains unknown. In this study,the Illumina sequencing method was used to examine the bacterial communities in micro-topographies,which are under the land cover of desert steppe in permafrost on the central Qinghai-Tibet Plateau. Three micro-morphologies(control,collapsing, slumped)were defined,and the relationships among bacterial community and environmental factors were investigated. The results showed that soil organic carbon contents in the collapsing and slumped areas were significantly lower than that in the control areas,and soil water contents were highest in the collapsing areas. For all the three types of soil,the most abundant bacterial phyla were Actinobacteria,Proteobacteria,Chloroflexi and Acidobacteria. Bacterial richness in collapsing area was higher than that in control area. Mantel test had found no significant relationships between bacterial community structures and soil physico-chemical properties. The Non-metric multidimensional scaling(NMDS)results showed that the bacterial community structure was significantly different among the three micro-morphologies. The results showed that in the desert steppe area,thaw slump changes the physical and chemical variables of the soil,further affects the relative abundance of specific bacteria at phylum level,while has no significant effect on the overall bacterial community structures.

Key words: thaw slump, Qinghai-Tibet Plateau, desert steppe, bacterial community, permafrost, high-throughput sequencing

中图分类号:

P343.6

张晓兰, 刘桂民, 李新星, 吴小丽, 徐海燕, 纪庚好, 李莉莎, 吴晓东. 青藏高原北麓河地区荒漠草原土壤细菌对热融滑塌的响应[J]. 冰川冻土, 2019, 41(4): 977-985.

ZHANG Xiaolan, LIU Guimin, LI Xinxing, WU Xiaoli, XU Haiyan, JI Genghao, LI Lisha, WU Xiaodong. The response of soil bacteria to thaw slump in a desert steppe in Beiluhe area, Qinghai-Tibet Plateau[J]. Journal of Glaciology and Geocryology, 2019, 41(4): 977-985.

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青藏高原北麓河地区荒漠草原土壤细菌对热融滑塌的响应

The response of soil bacteria to thaw slump in a desert steppe in Beiluhe area, Qinghai-Tibet Plateau

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