新疆东帕米尔高原慕士塔格峰洋布拉克冰川雪冰及融水中可培养细菌多样性分析

doi:10.7522/j.isnn.1000-0240.2015.0180

冰川冻土 ›› 2015, Vol. 37 ›› Issue (6): 1634-1641.doi: 10.7522/j.isnn.1000-0240.2015.0180

新疆东帕米尔高原慕士塔格峰洋布拉克冰川雪冰及融水中可培养细菌多样性分析

李明源^{1 2}, 王继莲², 古丽巴哈尔·萨吾提²

1. 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室, 新疆阿拉尔 843300;
2. 喀什大学叶尔羌绿洲生态与生物资源研究重点实验室, 新疆喀什 844006

收稿日期:2015-08-20 修回日期:2015-11-05 出版日期:2015-12-25 发布日期:2016-05-11
作者简介:李明源(1984-),男,甘肃民勤人,讲师,2012年在昆明理工大学获硕士学位,从事低温微生物学研究.E-mail:mingyuan_lee@yeah.net.
基金资助:
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题(BRYB1302);新疆维吾尔自治区高等学校科研计划项目(XJEDU2014S052)资助

Culturable bacterial diversity in snow, ice and meltwater of the Yangbark Glacier, Muztag Ata

LI Mingyuan^{1 2}, WANG Jilian², Gulbahar Sawut²

1. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Xinjiang Production & Construction Corps, Aral 843300, Xinjiang, China;
2. Key Laboratory of Ecology and Biological Resources in Yarkand Oasis of Education of Xinjiang Uygur Autonomous Region, Kashgar University, Kashgar 844006, Xinjiang, China

Received:2015-08-20 Revised:2015-11-05 Online:2015-12-25 Published:2016-05-11

摘要/Abstract

摘要： 采用LB和PYGV两种培养基分离培养慕士塔格峰洋布拉克冰川雪冰及融水中可培养细菌, 通过分离菌株16S rDNA的PCR-RFLP图谱和基于基因序列相似性的系统发育分析揭示细菌多样性. 结果从样品中共分离到178株菌, 分属于变形菌门(Proteobacteria)中的α-Proteobacteria、β-Proteobacteria、γ-Proteobacteria纲, 厚壁菌门(Firmicutes), 放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes)6个类群, 以假单胞菌Pseudomonas、黄杆菌Flavobacterium、紫色杆菌Janthinobacterium为优势菌群. LB和PYGV两种培养基在分离的细菌数量及种群多样性、优势菌属、特有菌属方面呈现一定差异. 结果表明, 慕士塔格峰洋布拉克冰川地区可培养细菌多样性丰富, 且存在一定的地方特异性菌属, 寡营养培养基更利于极端微生物的恢复生长.

关键词: 洋布拉克冰川, PCR-RFLP, 系统发育

Abstract: LB and PYGV culture medium were used to isolate the culturable bacterial in snow, ice and meltwater of the Yangbark Glacier, Muztag Ata, and the diversity of the isolates were analyzed based on the 16S rDNA PCR-RFLP and the phylogenetic analysis of 16S rRNA gene homology. The result indicated that a total of 178 bacterial strains were obtained, affiliated to six phyla as follows: α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, with Pseudomonas, Flavobacterium and Janthinobacterium strains being dominant. There were differences in bacterial number and diversity, dominant genera, specific genera between LB and PYGV culture medium. The results suggest that the glacier bacteria are abundant, some endemic genera exist in Yangbark Glacier, and the oligotrophic culture medium is more profitable for the growth of extreme microorganisms.

Key words: Yangbark Glacier, PCR-RFLP, phylogenetic analysis

中图分类号:

P938.1

李明源, 王继莲, 古丽巴哈尔·萨吾提. 新疆东帕米尔高原慕士塔格峰洋布拉克冰川雪冰及融水中可培养细菌多样性分析[J]. 冰川冻土, 2015, 37(6): 1634-1641.

LI Mingyuan, WANG Jilian, Gulbahar Sawut. Culturable bacterial diversity in snow, ice and meltwater of the Yangbark Glacier, Muztag Ata[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2015, 37(6): 1634-1641.

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新疆东帕米尔高原慕士塔格峰洋布拉克冰川雪冰及融水中可培养细菌多样性分析

Culturable bacterial diversity in snow, ice and meltwater of the Yangbark Glacier, Muztag Ata

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