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冰川冻土 ›› 2017, Vol. 39 ›› Issue (4): 781-791.doi: 10.7522/j.issn.1000-0240.2017.0088

• 冰冻圈与全球变化 • 上一篇    下一篇

天山乌源1号冰川表面冰尘及底部沉积层真菌群落结构比较及其系统发育分析

王叙贤1, 顾燕玲1, 倪雪姣1, 关波1, 倪永清1,2   

  1. 1. 石河子大学 食品学院, 新疆 石河子 832000;
    2. 中国科学院 西北生态环境资源研究院 冻土工程国家重点实验室, 甘肃 兰州 730000
  • 收稿日期:2017-01-06 修回日期:2017-06-05 出版日期:2017-08-25 发布日期:2017-11-15
  • 通讯作者: 倪永清,E-mail:niyqlzu@sina.com E-mail:niyqlzu@sina.com
  • 作者简介:王叙贤(1990-),女,山东烟台人,2012年在石河子大学取得学士学位,从事微生物生物信息学研究.E-mail:508996158@qq.com
  • 基金资助:
    国家自然科学基金项目(41271268;40961002;41140009);冻土工程国家重点实验室开放基金项目(SKLFSE200903);中国博士后科学基金项目(20100339)资助

Composition and phylogeny of fungal community in supraglacial cryoconite and subglacial sediments of the Glacier No.1 at headwaters of the Ürümqi River in Tianshan Mountains

WANG Xuxian1, GU Yanling1, NI Xuejiao1, GUAN Bo1, NI Yongqing1,2   

  1. 1. School of Food Sciences, Shihezi University, Shihezi 832000, Xinjiang, China;
    2. State Key Laboratory of Frozen Soil Engineering, Northwest Institute of Eco-Environmental Resources, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2017-01-06 Revised:2017-06-05 Online:2017-08-25 Published:2017-11-15

摘要: 对天山乌源1号冰川表面冰尘(Fz)及底部沉积层(Dz)的理化性质及真菌的多样性、群落结构、OTU的系统发育进行了比较分析。ITS克隆文库分析显示,依据99%相似性共得到26个真菌OTU,表面冰尘和底部沉积层分别为21和22个,其中17个为共有OTU。天山1号冰川中真菌以担子菌门(Basidiomycota)、子囊菌门(Ascomycota)占绝对优势,子囊菌门的真菌在Fz、Dz文库中的比例分别约为27%、57%。系统发育分析显示子囊菌门的真菌有14个OUT,8个属,其中Cladosporium仅分布在冰川表面(6.25%),而Aureobasidium仅出现冰川底部沉积层(8.93%)。另外6个属Tetracladium、Pseudeurotium、Fusarium、Penicillium、Simplicillium、Aspergillus在冰川底部和表面均有分布,但丰度明显不同,尤其是Aspergillus、Simplicillium在冰川底部沉积物中占优势(分别为17.86%、12.50%)。担子菌门的克隆主要来自Rhodotorula、Leucosporidium属,前者主要在分布冰川表面(12%),底部沉积层很少,后者在冰川表面及底部均有分布,分别占文库的比例为8.69%、12.50%。冰川表面和底部沉积层生态条件迥异,对其中的微生物系统发育类群具有明显的选择性。

关键词: 天山冰川, 冰尘, 沉积层, 真菌, 系统发育

Abstract: The diversity, composition and phylogeny of fungal community in supraglacial cryoconites and subglacial sediments of the Glacier No.1 in the headwaters of the Tianshan Mountains, China are assessed. Analysis of amplified rDNA ITS region sequences allows grouping of 203 clones into 26 operational taxonomic units (OTUs) using a cut-off of 99% identity. Twenty-two and twenty-one OTUs were found in the subglacial and supraglacial clone libraries, respectively. BLAST searches show that the subglacial community of the glacier was dominated by representatives of phylum Ascomycota (57% of clones), followed by phylum Basidiomycota (18% of clones) and Chytridiomycota (7% of clones), with the remainder of the library belonging to unclassified fungal OTUs (18% of clones). The supraglacial cryoconite sample had 27% Ascomycota, 22% Basidiomycota and 2.2% Chytridiomycota, and 49% of remaining library clones could not classified. Of the total 26 OTUs, 14 are related to Ascomycota cluster of sequences, including the genera Cladosporium, Aureobasidium, Tetracladium, Pseudeurotium, Fusarium, Penicillium, Simplicillium, Aspergillus. Cladosporium, which were absent in the subglacial sediment, were only present in supraglacial cryoconite sample. No clones related to Aureobasidium, a dominant group in the subglacial sediment (8.93%), were present in supraglacial cryoconite sample. The genera Aspergillus (17.86%) and Simplicillium (12.50%), which were rare in the supraglacial cryoconite library, were well represented in the subglacial sediment. In addition, Basidiomycota groups included relatives of Rhodotorula and Leucosporidium, of which the former well represented in the subglacial sediment (8.69%), the latter being the dominant group in the subglacial and supraglacial clone libraries. As a result, different glacial environments would harbor unique assemblages of microbes because they arose from different sources and/or undergo specific selective pressures in situ.

Key words: glaciers in the Tianshan Mountains, cryoconite, subglacial sediments, fungi, hylogenetics

中图分类号: 

  • Q938