青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征

doi:10.7522/j.issn.1000-0240.2018.1101

冰川冻土 ›› 2019, Vol. 41 ›› Issue (4): 968-976.doi: 10.7522/j.issn.1000-0240.2018.1101

青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征

王凤^1,5, 王宁练^2,4, 徐柏青^3,4, 沈亮³, 顾政权^1,5, 刘晓波¹

1. 中国科学院青藏高原研究所高寒生态学重点实验室, 北京 100101;
2. 西北大学城市与环境学院, 陕西西安 710069;
3. 中国科学院青藏高原研究所青藏高原环境变化与地表过程重点实验室, 北京 100101;
4. 中国科学院青藏高原地球科学卓越创新中心, 北京 100101;
5. 中国科学院大学, 北京 100049;
6. 中国科学院加德满都科教中心, 尼泊尔加德满都 44618

收稿日期:2018-07-25 修回日期:2019-01-18 出版日期:2019-08-25 发布日期:2019-11-28
通讯作者: 刘晓波,E-mail:xbliu@itpcas.ac.cn. E-mail:xbliu@itpcas.ac.cn
作者简介:王凤(1993-),女,山东枣庄人,2017年在曲阜师范大学(日照)获学士学位,现为中国科学院青藏高原研究所在读硕士研究生,从事冰芯微生物研究.E-mail:wangfeng@itpcas.ac.cn
基金资助:
国家自然科学基金项目（41425004）资助

Characteristics of the culturable bacteria in the surface and deep layers of the Tanggula Hariqin ice core,Tibetan Plateau

WANG Feng^1,5, WANG Ninglian^2,4, XU Baiqing^3,4, SHEN Liang³, GU Zhengquan^1,5, LIU Xiaobo¹

1. Key Laboratory of Alpine Ecology, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;
2. College of Urban and Environmental Science, Northwest University, Xi'an 710069, China;
3. Key Laboratory of Tibetan Environment Changes and Land Surface Process, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;
4. CAS Center for Excellence in Tibetan Plateau Earth Sciences, Beijing 100101, China;
5. University of Chinese Academy of Sciences, Beijing 100049, China;
6. Kathmandu Center for Research and Education, Chinese Academy of Sciences, Kathmandu 44618, Nepal

Received:2018-07-25 Revised:2019-01-18 Online:2019-08-25 Published:2019-11-28

摘要/Abstract

摘要： 对唐古拉哈日钦冰芯表层0.04~3.04 m及深层127.44~130.36 m中可培养细菌进行了对比研究。发现表层和深层可培养细菌数没有表现出显著差异性，表层冰芯中可培养细菌数为0~9.8×10³ CFU·mL^-1，略高于深层冰芯的0~8.4×10³ CFU·mL^-1。表层冰芯中微生物总数为10³~10⁶ cells·mL^-1，深层冰芯中微生物总数为10²~10³ cells·mL^-1。冰芯中可培养细菌属于Firmicutes（厚壁菌门）、Proteobacteria（变形菌门）、Actinobacteria（放线菌门）、Bacteroidetes（拟杆菌门）和Deinococcus-Thermus（异常球菌门） 5大类。但表层和深层可培养细菌属于不同的优势门，表层为Proteobacteria（38%），深层为Firmicutes（42%）。表层和深层优势属均为Bacillus（芽孢杆菌属），占比分别为18%和29%。已有研究表明哈日钦冰芯98.8 m达到了公元1000年的历史记录，因此对比千年尺度上可培养细菌数量及多样性的差异，能够为进一步发掘新基因，丰富微生物多样性，为了解可培养细菌的进化历史奠定基础。

关键词: 青藏高原, 哈日钦冰芯, 可培养细菌, 表层, 深层

Abstract: A comparative study was carried out on the culturable bacteria groups in the surface layer(0.04-3.04 m depth)and the deep layer(127.44-130.36 m depth)of the Tanggula Hariqin ice core. It was found that there was no significant difference in the number of culturable bacteria between the surface and deep layers. The number of culturable bacteria in the surface was 0-9.8×10³ CFU·mL^-1,slightly higher than that of deep ice core 0-8.4×10³ CFU·mL^-1. The total microorganisms cell count in the surface was 10³-10⁶ cells·mL^-1,while that in the deep layer was 10²-10³ cells·mL^-1. The culturable bacteria in ice core belonged to the five phyla, namely Proteobacteria,Firmicutes,Actinobacteria,Bacteroidetes and Deinococcus-Thermus. However,culturable bacteria at the surface and deep layer belonged to different dominant phylum,the surface is Proteobacteria (38%) and the deep layer is Firmicutes(42%). Nevertheless,Bacillus was the most dominant genus in both layers,with the abundance of 18% and 29% in surface and deep layers,respectively. The previous study shows the 98.8 m depth ice core of Hariqin reached 1000 A. D. historical record. Comparing the differences in the number and diversity of culturable bacteria on the millennium scale,it can further explore new genes,enrich microbial diversity and lay a foundation for understanding the evolution history of culturable bacteria.

Key words: Tibetan Plateau, Hariqin ice core, culturable bacteria, surface layer, deep layer

中图分类号:

Q939

王凤, 王宁练, 徐柏青, 沈亮, 顾政权, 刘晓波. 青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征[J]. 冰川冻土, 2019, 41(4): 968-976.

WANG Feng, WANG Ninglian, XU Baiqing, SHEN Liang, GU Zhengquan, LIU Xiaobo. Characteristics of the culturable bacteria in the surface and deep layers of the Tanggula Hariqin ice core,Tibetan Plateau[J]. Journal of Glaciology and Geocryology, 2019, 41(4): 968-976.

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青藏高原唐古拉哈日钦冰芯表层和深层可培养细菌特征

Characteristics of the culturable bacteria in the surface and deep layers of the Tanggula Hariqin ice core,Tibetan Plateau

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