山东滨海盐碱地柽柳林下微生物量变化特征及其影响因素

doi:10.7522/j.issn.1000-0240.2015.0059

冰川冻土 ›› 2015, Vol. 37 ›› Issue (2): 522-527.doi: 10.7522/j.issn.1000-0240.2015.0059

山东滨海盐碱地柽柳林下微生物量变化特征及其影响因素

刘万秋^1,2,3, 张威^1,2, 刘光琇^1,2, 章高森^1,2, 伍修锟^1,2, 陈熙明^1,2, 王筠^1,2

1. 中国科学院寒区旱区环境与工程研究所沙漠与沙漠化重点实验室, 甘肃兰州 730000;
2. 甘肃省极端环境微生物资源与工程重点实验室, 甘肃兰州 730000;
3. 昌邑海洋渔业局, 山东昌邑 261300

收稿日期:2014-12-16 修回日期:2015-03-15 出版日期:2015-04-25 发布日期:2015-06-06
通讯作者: 刘光琇, E-mail: liugx@lzb.ac.cn. E-mail:liugx@lzb.ac.cn
作者简介:刘万秋(1968-), 男, 山东昌邑人, 2015年在中国科学院寒区旱区环境与工程研究所获博士学位, 主要从事微生物生态学研究. E-mail: liuwq6803@163.com
基金资助:
国家自然科学基金项目(31470544); 海洋公益性行业科研专项经费项目(201505023)资助

The changing characteristics and influencing factors of the microbial biomass under shrub of Tamarix in Shandong coastal saline-alkali soil

LIU Wanqiu^1,2,3, ZHANG Wei^1,2, LIU Guangxiu^1,2, ZHANG Gaosen^1,2, WU Xiukun^1,2, CHEN Ximing^1,2, WANG Yun^1,2

1. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China;
2. Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou 730000, China;
3. Ocean and Fishery Bureau of Changyi, Changyi 261300, Shandong, China

Received:2014-12-16 Revised:2015-03-15 Online:2015-04-25 Published:2015-06-06

摘要/Abstract

摘要：

以山东昌邑国家级海洋生态特别保护区内柽柳林下土壤微生物为研究对象, 测定了其微生物量变化. 结果表明: 研究区细菌、真菌及古菌基因平均拷贝数分别为8.24×10⁶ copies·g^-1、1.51×10⁴ copies·g^-1和2.85×10⁴ copies·g^-1, 微生物量碳、氮平均值分别为140.54 mg·kg^-1和29.19 mg·kg^-1. 自密集区经稀疏区到边缘区, 随植被盖度的降低, 微生物量呈现降低的趋势. 相关分析表明, 不同植被盖度所造成的有机质输入的差异是造成这种变化规律的主要因素.

关键词: 滨海盐碱地, 柽柳, 定量PCR, 微生物量碳氮

Abstract:

Soil samples were collected under shrub of Tamarix in Marine Ecology Special Reserve, a national reserve in Changyi city of Shandong Province, to determine the microbial biomass. Results showed that ribosomal gene copies of bacteria, fungi, and archaea are 8.24×10⁶ copies·g^-1, 1.51×10⁴ copies·g^-1, 2.85×10⁴ copies·g^-1, respectively. The mean microbial biomass carbon and nitrogen are 140.54 mg·kg^-1 and 29.19 mg·kg^-1, respectively. From the dense zone, the sparse zone to the marginal zone, microbial biomass decreases with vegetation coverage. Correlation analysis indicates that the differences in organic matter input caused by vegetation coverage are the main factors for variance of microbial biomass. The establishment and growth of Tamarix provided stable growth conditions for soil microorganisms in the shallow layer of soil, resulting in similar microbial biomass under shrub of Tamarix from different habitats. Yet, there are large differences in microbial activity, e.g., a significant high microbial biomass carbon and nitrogen in costal saline-alkali soil than that in desert ecosystem.

Key words: coastal saline-alkali soil, Tamarix, quantitative PCR, microbial biomass carbon and nitrogen

中图分类号:

Q938.1

刘万秋, 张威, 刘光琇, 章高森, 伍修锟, 陈熙明, 王筠. 山东滨海盐碱地柽柳林下微生物量变化特征及其影响因素[J]. 冰川冻土, 2015, 37(2): 522-527.

LIU Wanqiu, ZHANG Wei, LIU Guangxiu, ZHANG Gaosen, WU Xiukun, CHEN Ximing, WANG Yun. The changing characteristics and influencing factors of the microbial biomass under shrub of Tamarix in Shandong coastal saline-alkali soil[J]. JOURNAL OF GLACIOLOGY AND GEOCRYOLOGY, 2015, 37(2): 522-527.

参考文献

[1] Shan Qihua, Zhang Jianfeng, Shen Liming, et al. Effects of forestry ecological engineering on herb community in coastal saline-alkali land[J]. Chinese Journal of Ecology, 2012, 31(6): 1411-1418. [单奇华, 张建锋, 沈立铭, 等. 林业生态工程措施对滨海盐碱地草本植物的影响[J]. 生态学杂志, 2012, 31(6): 1411-1418.]
[2] Chen Yingying, Fu Yuexin, Zhang Zhenke, et al. Review of the Chinese patents on coastal saline-alkali soil improvement[J]. Chinese Agricultural Science Bulletin, 2014, 30(11): 279-285. [陈影影, 符跃鑫, 张振克, 等. 中国滨海盐碱土治理相关专利技术评述[J]. 中国农学通报, 2014, 30(11): 279-285.]
[3] Jiang Zhimin, Chen Yuxia, Bao Ying. Genetic structure and population differentiation of Tamarix chinensis in Yellow River Delta, China[J]. Plant Diversity and Resources, 2011, 33(4): 403-408. [蒋志敏, 陈玉霞, 包颖. 黄河三角洲柽柳居群的遗传结构和遗传分化[J]. 植物分类与资源学报, 2011, 33(4): 403-408.]
[4] Saidana D, Mahjoub M A, Boussaada O, et al. Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae)[J]. Microbiological Research, 2008, 163(4): 445-455.
[5] Whiteman K E. Distribution of salt cedar (Tamarix spp. L) along an unregulated river in south-western New Mexico, USA[J]. Journal of Arid Environments, 2006, 64(2): 364-368.
[6] Wu Xiukun, Mao Wenliang, Tai Xisheng, et al. Progress in studies of microbial ecology in glacier foreland[J]. Journal of Glaciology and Geocryology, 2013, 35(1): 217-223. [伍修馄, 毛文梁, 台喜生, 等. 冰川前沿裸露地微生物生态学研究进展[J]. 冰川冻土, 2013, 35(1): 217-223.]
[7] Zong Meijuan, Zhang Mingcai, Song Baimin, et al. The study on microbial diversity and ecosystem function in Tamarix chinensis community of Yellow River Delta[C]//Biodiversity Conservation and Regional Sustainable Development in China: Proceedings of the Fourth National Symposium on the Conservation and Sustainable Use of Biodiversity in China. Beijing: China Forestry Publishing House, 2007: 347-352. [宗美娟, 张明才, 宋百敏, 等. 黄河三角洲柽柳群落土壤微生物多样性[C]//生物多样性保护与区域可持续发展: 第四届全国生物多样性保护与持续利用研讨会论文集. 北京: 中国林业出版社, 2007: 347-352.]
[8] Ma Xiaomei, Yin Linke, Chen Li. Study on vertical distribution of microorganismsin rhizosphere of populuseuphratica and Tamarix sp. in the lower reaches of the Tarim River, Xinjiang[J]. Arid Zone Research, 2008, 25(2): 183-189. [马晓梅, 尹林克, 陈理. 塔里木河干流胡杨和柽柳根际土壤微生物及其垂直分布[J]. 干旱区研究, 2008, 25(2): 183-189.]
[9] Lü Dongying, Li Weihong, Yang Yuhai, et al. Response of the quantity and distribution of microorganisms in the rhizosphere of Tamarix ramosissima seedlings to soil moisture[J]. Journal of Desert Research, 2012, 32(3): 737-743. [吕东英, 李卫红, 杨玉海, 等. 多枝柽柳幼苗根际微生物数量与分布对土壤水分变化的响应[J]. 中国沙漠, 2012, 32(3): 737-743.]
[10] Dong Kang, Li Shiweng, Kang Wenlong, et al. Study of the changes in microbe amounts and its affect factors in the soils along the Qinghai-Tibet Highway[J]. Journal of Glaciology and Geocryology, 2013, 35(2): 457-464. [董康, 李师翁, 康文龙, 等. 青藏公路沿线土壤微生物数量变化及其影响因素研究[J]. 冰川冻土, 2013, 35(2): 457-464.]
[11] Yong Xiaoyu, Cui Yaqing, Chen Lihua, et al. Dynamics of bacterial communities during solid-state fermentation using agro-industrial wastes to produce poly-γ-glutamic acid, revealed by real-time PCR and denaturing gradient gel electrophoresis (DGGE)[J]. Applied Microbiology and Biotechnology, 2011, 92(4): 717-725.
[12] Prevost-Boure N C, Christen R, Dequiedt S, et al. Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR[J]. PLoS One, 2011, 6(9): e24166.
[13] Einen J, Thorseth I H, vre s L. Enumeration of Archaea and Bacteria in seafloor basalt using real-time quantitative PCR and fluorescence microscopy[J]. FEMS Microbiology Letters, 2008, 282(2): 182-187.
[14] Chen ming, Zhu Jianwen, Sheng Jiandong, et al. The effect of Tamarix spp canopy on the soil enzyme activities and the microbial quantity[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(2): 212-217. [陈明, 朱建雯, 盛建东, 等. 柽柳冠茎对其土壤酶活性及微生物数量的影响[J]. 西北农业学报, 2008, 17(2): 212-217.]
[15] Zhao Xianli, Zhou Guangsheng, Lü Guohong. The study on soil microbial characteristic under different types of vegetation in Liaohe Delta[J]. Chinese Journal of Soil Science, 2009, 40(6): 1266-1269. [赵先丽, 周广胜, 吕国红. 辽河三角洲不同植被类型土壤微生物特征研究[J]. 土壤通报, 2009, 40(6): 1266-1269.]
[16] Yang Gang, He Xunyang, Wang Kelin, et al. Effects of vegetation types on soil micro-biomass carbon, nitrogen and soil respiration[J]. Chinese Journal of Soil Science, 2008, 39(1): 189-191. [杨刚, 何寻阳, 王克林, 等. 不同植被类型对土壤微生物量碳氮及土壤呼吸的影响[J]. 土壤通报, 2008. 39(1): 189-191.]
[17] Zhu Hong, Zhao Chengyi, Li Jun, et al. Distribution characteristics of soil MBC and relevant factors in woodland of Tamarlx ramasissima, central Asia[J]. Acta Pedologica Sinica, 2008, 45(2): 375-379. [朱宏, 赵成义, 李君, 等. 柽柳林地土壤微生物量碳及相关因素的分布特征[J]. 土壤学报, 2008, 45(2): 375-379.]
[18] Yao Yuan, Ding Jianli, Lei Lei, et al. Monitoringspatial variabilityof soil salinityindry and wet seasons in the north Tarim Basin using remote sensing and electromagnetic induction instruments[J]. Acta Ecologica Sinica, 2013, 33(17): 5308-5319. [姚远, 丁建丽, 雷磊, 等. 干湿季节下基于遥感和电磁感应技术的塔里木盆地北缘绿洲土壤盐分的空间变异性[J]. 生态学报, 2013, 33(17): 5308-5319.]
[19] Qin lu, Lü Guanghui, He Xuemin. Effects of freezing-thawing on soil microbial quantity and community structure around the Ebinur Lake[J]. Journal of Glaciology and Geocryology, 2013, 35(6): 1590-1599. [秦璐, 吕光辉, 何学敏. 艾比湖地区冻融作用对土壤微生物数量和群落结构的影响[J]. 冰川冻土, 2013, 35(6): 1590-1599.]
[20] Lei Jinyin, Ban Nairong, Zhang Yonghong, et al. Effects and partitioncharacteristics of Tamarix ramosissima on nutrients and salt of saline-alkali soils[J]. Bulletin of Soil and Water Conservation, 2011, 31(2): 73-76. [雷金银, 班乃荣, 张永宏, 等. 柽柳对盐碱土养分与盐分的影响及其区化特征[J]. 水土保持通报, 2011, 31(2): 73-76.]
[21] He Jizheng, Shen Jupei, Zhang Limei. Advance in the research of soil non-thermophilic Crenarchaeota[J]. Acta Ecologica Sinica, 2009, 29(9): 5047-5055. [贺纪正, 沈菊培, 张丽梅. 土壤中温泉古菌研究进展[J]. 生态学报, 2009, 29(9): 5047-5055.]
[22] Liu Fan, Ye Siyuan, Tang Yueqin, et al. Analysis of microbial community structurein coastal wetland soil of the Yellow River Delta[J]. Chinese Journal of Applied Environmental Biology, 2007, 13(5): 691-696. [刘芳, 叶思源, 汤岳琴, 等. 黄河三角洲湿地土壤微生物群落结构分析[J]. 应用与环境生物学报, 2007, 13(5): 691-696.]
[23] Liu Guangxiu, Dong Xiaopei, Zhang Wei, et al. The changing mechanisms of microbial number on surface soil with altitude[J]. Journal of Glaciology and Geocryology, 2010, 32(6): 1170-1174. [刘光琇, 董小培, 张威, 等. 不同海拔表层土壤微生物数量消长的机理[J]. 冰川冻土, 2010, 32(6): 1170-1174.]

山东滨海盐碱地柽柳林下微生物量变化特征及其影响因素

The changing characteristics and influencing factors of the microbial biomass under shrub of Tamarix in Shandong coastal saline-alkali soil

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