Biying CHONG, Xianling JIN, Haiyan XU, Xiaodong WU, Wenting ZHOU, Bingyan LIANG, Zipeng GAN, Ting ZHANG, Guohui KANG, Qiuli SHU, Zhuo CHEN, Guimin LIU
In the context of climate warming, fencing is a common management method for grassland ecosystems. Understanding the effects of warming and fencing on soil organic carbon accumulation is very important to evaluate the carbon source and sink roles of grassland ecosystems. In this study, using the amino sugars as biomarkers, we investigated the effects of long-term warming and fencing on necromass accumulation in an alpine grassland. We collected soil samples from plots after 15 years treatment of warming and fencing. The results showed that: (1) Compared to the control, there was no significant difference in the carbon source derived from bacteria and fungi between the warming + enclosure and enclosure treatments, but bacterial residual carbon contributed more to soil organic carbon than fungal residual carbon. (2) Compared to the control, there was no significant effect on the total amount of amino sugars between the warming + enclosure and enclosure treatments, but significantly reduced the long-term turnover of microbial carbon in the soil, and the short-term turnover was not significant. (3) Total amino sugars, fungal necromass carbon and bacterial necromass carbon were significantly positively correlated with conductivity, moisture content, soil organic carbon (SOC), and total nitrogen (TN). In summary, bacterial necromass carbon contributed higher proportions to soil organic carbon than fungal necromass carbon, while 15 years warming and fencing had no significant effects on microbial necromass carbon in soils.